2021 J Neurochem 158(3):640-656. Cannabinoid Receptor 1 associates to different molecular complexes during GABAergic neuron maturation.

We studied the interactome of CB1 receptor by proteomics during GABA neuron maturation. We describe new partners of this receptor that may be relevant to understand cannabinoid actions on neuronal development (DLAT, FASN, YWHAZ, VDAC1, MPRIP, USHBP1) and that the interactome of CB1 is enriched at the more immature stage in mitochondrial associated proteins and metabolic molecular functions, whereas, at a more mature stage, CB1 complex is enriched in synaptic-associated proteins. We also described different partners depending on the use of N-terminal or C-terminal directed antibodies, suggesting different interactomes associated to receptor states .